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Lynch Lab

Mechanisms and Consequences of Regulated Alternative Splicing
  • Home
  • Research
  • Publications
  • Protocols
  • People
  • Contact
  • Intranet
  • Penn RNA Group

Lynch Lab Protocols

Click on a protocol to view a PDF:

Making Hot RNA Probe

JSL1 Culturing, Stimulation, and Freezing

Linearizing DNA for Transfection

Care and Feeding for mAb104 Cells

Radioactive Marker Protocol

Recipes

RNA Affinity Purification

Isolating RNA with RNABee

RT-PCR (General)

Transfections into JSL1 Cells for Stable Cell Lines

Transformation of Competent DH5alpha

UV Crosslinking

Western Blots with ECL

Rules for Primer Design

 

If you have any questions please feel free to contact us.

 

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klync@pennmedicine.upenn.edu

phone: 215-573-7749